Nek2 Antibody (D-8): sc-55601

Nek2 Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Nek2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Nek2 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nek2 plays a crucial role in cell cycle regulation, particularly during the transition from G2 phase to mitosis, where expression peaks. This regulation is vital for proper cell division, as aberrations in Nek2 activity can lead to genomic instability and contribute to tumorigenesis. Additionally, Nek2 is closely related to NIMA protein found in Aspergillus nidulans, which is essential for mitotic entry, highlighting evolutionary conservation of function. In mammalian systems, Nek2 expression is tightly regulated, and dysregulation has been implicated in various cancers, making Nek2 a significant target for research and therapeutic interventions. Nek2 (D-8) antibody effectively detects across multiple species and applications, making this tool invaluable for researchers studying cell cycle dynamics and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nek2 Antibody (D-8) References:

1. Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators.  |  Tanaka, K. and Nigg, EA. 1999. J Biol Chem. 274: 13491-7. PMID: 10224116
2. NIMA-related kinases: isolation and characterization of murine nek3 and nek4 cDNAs, and chromosomal localization of nek1, nek2 and nek3.  |  Chen, A., et al. 1999. Gene. 234: 127-37. PMID: 10393247
3. A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells.  |  Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
4. Mitotic induction and maintenance by overexpression of a G2-specific gene that encodes a potential protein kinase.  |  Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
5. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.  |  Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
6. The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes.  |  Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
7. Characterization of mammalian NIMA-related kinases.  |  Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nek2 Antibody (D-8) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-Nek2 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nek2 Antibody (D-8) Specifications

Alternate Product Name: Nek2

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 211-445

Epitope Target: Nek2

Epitope Species: human

Product Reactivity/Detection: Nek2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for Nek2 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin